Analysis Note

ControlFetal heart

Application

Western blot: 1-10 ug/mL using ECL. Expected size of Ang-1 is ~55kDa.

ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).

Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.

Optimal working dilutions must be determined by end user.

This Anti-Angiopoietin-1 Antibody, N-terminus is validated for use in ELISA, WB for the detection of Angiopoietin-1.

Research CategoryCell Structure

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development. Angiopoietin-1 (mouse and human Ang-1; 498aa with 98% identity) is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic dvevelopment, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496aa with 85% identity) may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.

Immunogen

20 amino acid peptide sequence near the amino-terminus of mouse Ang-1.

Epitope: N-terminus

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

Specificity

Recognizes mouse Angiopoietin-1 (Ang-1). The immunogenic peptide sequence is 90% (18/20 aa) conserved in human Ang-1, 95% I pig and bovine, and has no significant sequence homology with Ang-2. Antibody crossreactivity in other species is not established.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

55 kDa